Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abno

Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment

Xiao-Lan Chen;Jing-Gen Liu;Gang Lu;Ying-Xia Gong;Liang-Cai Zhao;Jie Chen;Zhi-Qiang Chi;Yi-Ming Yang;Zhong Chen;Qing-lin Li

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2007(017)008

【摘要】Dependence and impairment of learning and memory are two well-established features caused by abused drugs such as opioids. The hippocampus is an important region associated with both drug dependence and learning and memory. However, the molecular events in hippocampus following exposure to abused drugs such as opioids are not well understood. Here we examined the effect of chronic morphine treatment on hippocampal protein expression by proteomic analyses. We found that chronic exposure of mice to morphine for 10 days produced robust morphine withdrawal jumping and memory impairment, and also resulted in a significant downregulation of hippocampal protein levels of three metabolic enzymes, including Fe-S protein 1 of NADH dehydrogenase, dihydrolipoamide acetyltransferase or E2 component of the pyruvate dehydrogenase complex and lactate dehydrogenase 2. Further real-time quantitative PCR analyses confirmed that the levels of the corresponding mRNAs were also