Cloning and Characterization of Genes Encoded in 187 dTDP-D-mycaminose Biosynthetic Pathwa

Cloning and Characterization of Genes Encoded in 187 dTDP-D-mycaminose Biosynthetic Pathway

from a Midecamycin-producing Strain,Streptomyces mycarofaciens

Lina CONG;Wolfgang PIEPERSBERG

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2007(039)003

【摘要】Two subclusters from Streptomyces mycarofaciens,a midecamycin producer,were cloned and partially sequenced.One region was located at the 5'end of the mid polyketide synthase (PKS) genes and contained the genes midA,midB and midC.The other region was at the 3'end of the PKS genes and contained midK,midI and midH.Analysis of the nucleotide sequence revealed that these genes encode dTDP-glucose

synthase

(midA),dTDP-glucose (midC),methyltransferase

dehydratase(midB),aminotransferase

(midK),glycosyltransferase(midI)and an assistant gene(midH).All of these genes are involved in the biosynthesis of dTDP-D-mycaminose,the first deoxysugar of midecamycin,and in transferring the mycaminose to the midecamycin aglycone in S.mycarofaciens.Similar to gene pairs des VIII/des VII in S.venezuelae and tylMIII/tylMII in S.fradiae,the product of midH probably functions as an auxiliary protein required by the MidI protein for efficient glycosyltransfer in midecamycin biosynthesis.

【总页数】7页(187-193)

【关键词】deoxysugar;dTDP-D-mycaminose;midecamycin;Streptomyces mycarofaciens

【作者】Lina CONG;Wolfgang PIEPERSBERG

【作者单位】College of Biology and Food Technology,Dalian Institute of Light

Industry,Dalian

116034,China;Institute

of

Chemical

Microbiology,Bergische University,D-42097 Wuppertal,Germany 【正文语种】中文 【中图分类】Q5 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_acta-biochimica-biophysica-sinica_thesis/0201254943539.html 【相关文献】

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