A Novel Porcine Model of Septic Shock Induced by Acute Respiratory Distress Syndrome due t

A Novel Porcine Model of Septic Shock Induced by

Acute Respiratory Distress Syndrome due to Methicillin-resistant Staphylococcus aureus

Shuo Wang;Jun-Yu Wang;Tao Wang;Chen-Chen Hang;Rui Shao;Chun-Sheng Li

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2017(130)010

【摘要】Background:Sepsis is one of the main causes of mortality in critically ill patients following progression to septic shock.To investigate the pathophysiologic changes of sepsis,we developed a novel porcine model of septic shock induced by acute respiratory distress syndrome (ARDS) due to methicillin-resistant Staphylococcus aureus (MRSA) pneumonia.Methods:Twenty-six

male

Landraces

(Lvyuanweiye,Beijing,China) weighing 30 ± 2 kg were divided into four groups:sham group (SH;n =5);cotton smoke inhalation group (SM;n =6);MRSA pneumonia group (MR;n =6);and septic shock group with cotton smoke inhalation + MRSA pneumonia (SS;n =9).Extensive hemodynamics,oxygen dynamics,and lung function were monitored for 24 h following the injury or until death.Tissues were collected,and histopathology evaluations were carried out.Results:Blood cultures from 6 of 9 animals in the SS group were positive for MRSA.Two hours following the injury,decreased mean arterial blood pressure (60-70 mmHg) and cardiac index (＜2 L.min-1 ·m-2) were observed in the