RP-HPLC同时测定华山参滴丸中3种有效成分的含量
董林毅;张韻慧;李若洁;侯媛媛
【期刊名称】《中国药学杂志》 【年(卷),期】2011(046)005
【摘要】目的:建立反相高效液相色谱法同时测定华山参滴丸中3种有效成分的含量.方法:采用Phenomenex Luna C(18)色谱柱(4.6 mm×250 mm,5μm),流动相为20 mmol·L(-1)醋酸铵水溶液-甲醇(70:30),流速为1.0 mL·rain(-1),检测波长215 nm,进样量20μL.结果:硫酸阿托品、氢溴酸东莨菪碱和消旋山莨菪碱的
线
性
范
围
分
别
为
1.444-28.88μg(r=0.9999),0.891-17.82
μg(r=0.9999),2.196-43.92μg(r=0.9999);平均回收率(n=6)分别为101.82%(RSD=2.89%)、100.17%(RSD:2.49%)、98.68%(RSD=2.33%).结论:所建立的RP-HPLC方法简便、准确、快速、重复性好,可用于华山参滴丸中硫酸阿托品、氢溴酸东莨菪碱和消旋山莨菪碱3个有效成分的含量测定.%OBJECTIVE To develop a RP-HPLC method for determination of three components ( atropine sulfate, scopolamine hydrobromide and raceanisodamine) in Huashanshen dripping pills. METHODS The analysis was performed on a Phenomenex Luna C18 column (4. 6 mm × 250 mm ,5 μm)with the mixture of methanol and 20 mmol · L- 1ammonium acetate buffer solution (30: 70) as mobile phase, and the flow rate was 1.0 mL · min-1. The detection wavelength was 215 nm. The column temperature was room temperature(25 ℃ ). RESULTS The calibration curves of atropine sulfate, scopolamine hydrobromide and
RP-HPLC同时测定华山参滴丸中3种有效成分的含量
