Observation of nuclei reassembled from demembranated Xenopus sperm nuclei and analysis of

Observation of nuclei reassembled from demembranated Xenopus sperm nuclei and

analysis of their lamina components

QUJIAN;CHUANMAOZHANG;等

【期刊名称】《细胞研究：英文版》 【年(卷),期】1994(004)002

【摘要】A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei.Electron microscopy revealed that the reassembled nucleus had a double-layered nuclear memblane,nuclear pore complexes,and decondensed chromatin etc.Indirect immunofluorescence analysis demonstrated the presence of lamina in newly assembled nuclei.Western-blotting results showed that lamin LII was present in egg extracts and in lamina of the reassembled nuclei which were previously reported to contain only egg derived lamin LIII.

【总页数】10页(163-172)

【关键词】爪蟾;精子;细胞核;去膜;体外核装配;结构分析;核被膜;核纤层 【作者】QUJIAN;CHUANMAOZHANG;等 【

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CollegeofLifeSciences,BeijingUniversity,Beijing100871,China.;CollegeofLifeSciences,BeijingUniversity,