The effect of MAGE-A1 gene on NIH3T3 cells
Jingjun
Sun;Jin
Zhu;Zhenning
Qiu;Yuhua
Li;Guipeng
Ding;Yi
Zhu;Zhenqing
Feng;Xiaohong Guan
【期刊名称】《生物医学研究杂志（英文版）》 【年(卷),期】2005(019)003
【摘要】Objective: Melanoma antigen genes(MAGE) genes have been found in many kinds of tumor tissue, but not in normal tissue except testis and placentas. The Ags encoded by MAGE genes therefore are strictly tumor-specific. The most current researches associated with these genes focus on the tumor vaccination using these Ags. Few reports are concerning these genes' functions. In this study, we investigated the role of MAGE-A1 gene on NIH3T3 cells after transferring with it. Methods: Clone the MAGE-A1 into the plasmids pEGFP-C3 and pcDNA3.1, then transfer the reconstructed plasmids and primary plasmids into the NIH3T3 cells using a new transfer reagent FuGENE 6. Selecting the positively transferred cells by G418. Identified by RT-PCR, Western blot, Immunocytochemistry,Laser Scanning Confocal Microscope and Fluoroscope. The cells mobile ability was measured with Millicell-PCF. The cell cycle and apoptosis were measured with Flow Cytometry. Results: The apoptosis rate of NIH3T3 cells that transferred with control plasmid pcDNA3.1was 13.4% and the raitos that stay in S phase and G2-M phase were 5.68% and 1.04%
The effect of MAGE-A1 gene on NIH3T3 cells
