Effect of moxibustion on long-term memory in
vascular dementia model rats
Yue LI;Jun YANG;Li GUI;Yin-qiu FAN
【期刊名称】《世界针灸杂志（英文版）》 【年(卷),期】2019(029)001
【摘要】Objective:To observe the effects on spatial long-term memory in the rats with vascular dementia (VD) treated with moxibustion for resolving stasis and promoting meridian circulation.Methods:The modified 2-vascular occlusion (2-VO) method was used to prepare VD animal model.The rats were randomized into a sham-operation group,a model group,a moxibustion group and a western medication group,15 rats in each one.In the moxibustion group,mild warm moxibustion was applied to “Bǎihuì(百会GV 20)\GV 14)\and \庭GV24)\at each point,once a day,for 3 weeks consecutively.In the western medicaion group,mouse nerve growth factor (NGF) was injected
intraperitoneally,0.18
mL/kg,once
a
day,for
3
weeks
consecutively.Morris water maze test was used,the time of the first passing platform,the frequency of passing-platform and swimming speed in 120s were determined of the rats in each group.Western blot were used to test the protein expressions of hippocampal Nestin and DCX.Results:At the end of three courses of treatment,compared with sham-operation group,the difference was not significant statistically in
swimming speed in the model group,the moxibustion group and the western medication group separately (all P＞ 0.05).Three days after modeling,the mean of escape latency was prolonged obviously in the modeled rats compared with the rats in the shamoperation group (88.84±19.94 vs 18.15±9.41,P＜0.01).At the end of three courses of treatment,compared with the sham-operation group(22.01 ± 10.07),the mean of escape latency was different statistically in the model group(89.18 ±19.70),the moxibustion group(37.21 ±13.31) and the western
medication
group
separately(51.50
±16.15),all
P
＜
0.01.Compared with the model group,the mean of escape latency was shortened in the moxibustion group and western medication group respectively (37.21 ±13.31 vs 89.18 ±19.70,51.50 ±16.15 vs 89.18 ± 19.70,both P ＜ 0.01).Compared with the western medication group,there was no statistical significant difference in the mean of escape latency in the moxibustion group (37.21±13.31 vs 51.50±16.15,P＞0.05).Compared with the sham-operation group,the frequency of passing-platform was decreased in the model group (0.73 ±0.96 vs 2.60±1.45,P＜ 0.01);compared with model group,the frequency of passing-platform was increased in the moxibustion group and the western medicine group and the statistically significant difference presented in the moxibustion group (2.06 ±1.33 vs 0.73 ± 0.96,P ＜ 0.01).Compared with the sham-operation group,the time of first
passing-platform was prolonged in the model group (87.86 ±33.25 vs 36.13 ± 29.76,P＜ 0.01).Compared with the model group,the time of first passing-platform was shortened in the moxibustion group and the western medicine group respectively (49.53 ±29.48 vs 87.86 ± 33.25,P ＜ 0.01;58.98 ± 36.22 vs 87.86 ± 33.25,P ＜ 0.05).After three courses of treatment,compared with the sham-operation group,Nestin expression was reduced in the model group,(0.33 ±0.12 vs 0.51 ± 0.02,P ＜ 0.05) and was increased in the moxibustion group and the western medication group respectively (1.33 ±0.17 vs 0.51 ± 0.02,1.39 ± 0.10 vs 0.51 ± 0.02,both P ＜ 0.01).Compared with the model group,Nestin expression was increased in the moxibustion group and the western medication group,respectively (1.33 ± 0.17 vs 0.33 ± 0.12,1.39 ± 0.10 vs 0.33 ± 0.12,both P ＜ 0.01).Compared with the western medication group,the difference was not significant statistically in the mox-ibustion group (1.33±0.17 vs 1.39±0.10,P＞ 0.05).After three courses of treatment,compared with the sham-operation group,DCX expression was decreased in the model group,but without significant difference (0.44±0.20vs 0.51 ±0.26,P＞ 0.05),the expression was increased in the moxibustion group and the western medication group respectively (0.98 ± 0.25 vs 0.51 ± 0.26,P ＜ 0.05;1.11 ± 0.43 vs 0.51 ±0.26,P＜0.01).Compared with the model group,DCX expression was obviously increased in the moxibustion group and the western medication group
Effect of moxibustion on long-term memory in vascular dementia model rats
