不同浓度低氧暴露对慢性疲劳综合征建模小鼠血清IL-6、
TNF-α及IL-1β水平的影响
张亚军;王茹;P.R.Mabounda Kounga;王红霞;李丽辉;陈佩杰
【期刊名称】《中国运动医学杂志》 【年(卷),期】2013(032)006
【摘要】Objective To study the effect of hypoxia exposure on the levels of pro-inflammatory cytokine(IL-6,TNF-α,and IL-1β) in mice with chronic fatigue syndrome(CFS).Methods 72 ICR male mice were randomly divide into 6 groups:normal group (C0),CFS group (CFS0),normal without hypoxia exposure group(C),C FS without hypoxia exposure group (CFS),CFS with 15％O2 hypoxia exposure group (15％H),and CFS with 18％O2 hypoxia exposure group (18％H).CFS of the mice was induced by restraint and forced swimming,and the normal mice were fed under the same conditions.Groups 15％H and 18％H received hypoxia exposure one hour per day for seven days.The blood samples were collected to detect serum IL-6,TNF-α,and IL-1β by enzyme.Results (1)Compared to group C0,plasma IL-6,TNF-α,and IL-1β levels in group CFS0 significantly increased
after
4
weeks
restraint
and
forced
swimming
stress.(2)Compared to group CFS,group 15％H exhibited a decreasing trend in IL-6 and IL-1β levels,and there was no significant difference in the IL-6 level between groups 15％H and C,but the IL-1β level was still higher than group C; No significant difference was observed in the IL-6
and IL-1β levels between groups 18％H and CFS,and the IL-6 and IL-1β levels in group 18％H were still higher than group C.No significant difference was found in TNF-α level among groups C,CFS,18％H and 15％ H.Conclusion Four-week restraint and forced swimming stress successfully induces increase in proinflammation cytokines in mice with chronic fatigue syndrome.Moreover,hypoxia exposure one hour per day for seven days is beneficial for regaining pro-inflammation cytokines IL-6 to normal level.%目的:探讨不同浓度低氧暴露对慢性疲劳综合征(CFS)小鼠外周血促炎因子IL-6、TNF-α及IL-1β水平的影响.方法:雄性ICR小鼠72只,随机分为6组,每组12只,即建模正常对照组(C0组)、慢性疲劳综合征建模组(CFS0组)、低氧暴露正常对照组(C组)、低氧暴露慢性疲劳综合征对照组(CFS组)、慢性疲劳综合征+15％氧浓度低氧暴露组(15％H组)和慢性疲劳综合征+18％氧浓度低氧暴露组(18％H组),其中CFS0组、CFS组、15％H组和18％H组采用束缚和强迫游泳方式建立慢性疲劳综合征小鼠模型,C0组和C组同等条件下正常饲养.建模完成后C0组和CFS0组立即处死,检验建模效果;C组继续饲养7天,CFS组自然恢复7天,15％H组和18％H组分别在15％和18％氧浓度环境暴露7天(1小时/天),之后处死各组小鼠,检验低氧暴露效果.采用酶联免疫法检测各组小鼠外周血促炎因子IL-6、TNF-α及IL-1β水平.结果:(1)CFS建模完成后,CFS0组小鼠外周血促炎因子IL-6、TNF-α及IL-1β水平较C0组显著升高(P＜0.05).(2)低氧干预后,15％H组外周血IL-6和IL-1β水平较CFS组呈下降趋势(P＞0.05),IL-6较C组无显著性差异,但IL-1β仍显著高于C组(P＜0.05);18％H组小鼠外周血IL-6和IL-1β水平与CFS组无显著性差异(P＞0.05),
仍明显高于C组(P＜0.05);CFS组小鼠外周血IL-6和IL-1β水平仍显著高于C组(P＜0.05),但各组间TNF-α水平无显著性差异(P＞0.05).结论:通过束缚和强迫游泳成功建立以外周血促炎因子IL-6、TNF-α和IL-1β升高为特征的慢性疲劳综合征小鼠模型,而在氧浓度为15％环境中暴露7天(1小时/天)有助于慢性疲劳综合征小鼠外周血促炎因子IL-6恢复到正常水平. 【总页数】7页(507-513)
【关键词】慢性疲劳综合征;低氧;慢性炎症;免疫紊乱
【作者】张亚军;王茹;P.R.Mabounda Kounga;王红霞;李丽辉;陈佩杰
【作者单位】运动健身科技省部共建教育部重点实验室,上海体育学院 上海200438;运动健身科技省部共建教育部重点实验室,上海体育学院 上海200438;运动健身科技省部共建教育部重点实验室,上海体育学院 上海200438;运动健身科技省部共建教育部重点实验室,上海体育学院 上海200438;运动健身科技省部共建教育部重点实验室,上海体育学院 上海200438;运动健身科技省部共建教育部重点实验室,上海体育学院 上海200438 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-sports-medicine_thesis/0201240009349.html 【相关文献】
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不同浓度低氧暴露对慢性疲劳综合征建模小鼠血清IL-6、TNF-α及IL-1β
