Recombinant Vaccinia Virus is an Effective and Non-perturbing Vector for Human Dendritic C

Recombinant Vaccinia Virus is an Effective and Non-perturbing Vector for Human Dendritic Cells

Transfected with Epstein-Barr Virus Latent

Membrane Protein 2A

许继军; 姚堃; 彭光勇; 谢芳艺; 丁传林; 朱建中; 秦健

【期刊名称】《《生物医学研究杂志（英文版）》》 【年(卷),期】2002(016)001

【摘要】ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein-Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investigation on the therapeutic vaccines against EBV-associated malignancies.MethodsMature DC were transfected with EBV-LMP2A recombinant vaccinia virus (rVV-LMP2A).Before and after the transfection,the expression of surface antigens on mature DC including CD1a,CD83,CD40,CD80,HLA-DR

was

measured

by

fluorescence

activated cell sorter (FACS) and the function of DC to stimulate allogeneic T cells proliferation was measured by mixed leukocyte reactions (MLR).ResultsLMP2A protein was highly expressed (66.1%) in DC after the transfection of rVV-LMP2A.No significant changes in the primary surface antigens expression and in the MLR were detected during the transfection.Transfected DC still had strong potential in stimulating

the

proliferation

of

allogeneic

T