烧伤患者耐甲氧西林金黄色葡萄球RAPD分析
石书凡;杨长顺
【期刊名称】《医学信息》 【年(卷),期】2014(000)024
【摘要】目的：调查烧伤患者耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及分子流行病学情况,为控制感染提供科学依据。方法烧伤患者分离的金黄色葡萄球菌用PCR法扩增其mecA基因确定MRSA菌株,K-B纸片法检测MRSA的耐药性,随机引物扩增多态性DNA(RAPD)进行同源性分析。结果 MRSA的分离率为41.1%,对多种抗生素均耐药,没有发现耐万古霉素和替考拉宁的菌株。23株MRSA经RAPD分型,均获得稳定的条带,可分为四型,以Ⅱ型为主,共检出13株。结论烧伤患者分离MRSA菌株具有多重耐药性,应根据药敏试验结果合理选用抗生素进行治疗；采用RAPD对MRSA的同源性进行研究,为控制感染提供分子流行病学依据。%Objective To investigate the status of antibiotics resistance and the genetic homology of methicil in-resistant staphylococcus aureus isolated from burn wounds and provide scientific evidence for control ing MRSA infection. Methods MRSA was identified by mecA PCR. Drug resistance was perfomed using K-B method. The RAPD technique was used to identify the genotypes. Results The separation rate of MRSA was 41.1%. MRSA was resistant to much drugs .But al strains were susceptible to vancomycin and teicoplanin. All 23 stains of MRSA produced fingerprints by RAPD, and was classified into four genotypes.13 strains wereIItype which was the dominant type.
烧伤患者耐甲氧西林金黄色葡萄球RAPD分析
