Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs

Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs

佚名

【期刊名称】《自然科学进展（英文版）》 【年(卷),期】2002(012)006

【摘要】cDNA libraries from aborted human 3-month fetal brain,adult rat and mouse brain were constructed by using a yZAP express cDNA library construction kin.Low molecular weight fragments of the second strand cDNASA were removed by flowing through the Sepharose CL-4B column and the frractionated long,Middle,Short fragments and the combined fragments weire respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus.The 5'ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced.A total of 894 ESTs were obtained and some full-length clones were squenced.By andalyaing the se-quences,12 novel full-length cDNAs were obtained. 【总页数】4页(477-480)

【关键词】cDNA library;brain;EST 【作者】佚名

【作者单位】State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical SDciences & Peking Union Medical College,Chinese National Human Genome