PEI-Fe3O4纳米颗粒标记人羊膜上皮细胞
张殿宝;李莹;苏航;王竟;施萍;庞希宁
【期刊名称】《中国医学科学院学报》 【年(卷),期】2017(039)005
【摘要】目的 探索使用PEI-Fe3O4纳米颗粒标记人羊膜上皮细胞(hAEC).方法 采用透射电子显微镜和动态光散射表征PEI-Fe3O4纳米颗粒.使用纳米颗粒标记原代培养的hAEC,普鲁士蓝染色评价其标记效率,台盼蓝染色法和CCK-8法检测细胞存活率和细胞毒性.结果 PEI-Fe3O4纳米颗粒呈紧凑的球形,平均粒径为13 nm,水动力学半径为17.56nm,zeta电位为+34.5 mV.浓度大于20μg/ml的纳米颗粒对hAEC的标记效率达到91％.50(t=16.37,P＜0.0001;t=10.39,P＜0.0001)、100 μg/ml(t=29.89,P＜0.0001;t=16.86,P＜0.0001)纳米颗粒浓度下的hAEC存活率和细胞活力均明显低于未标记时.结论 PEI-Fe3O4纳米颗粒可用于标记hAEC,在其工作浓度下未表现出显著的细胞毒性.%Objective To label human amniotic epithelial cells (hAECs) by using PEI-Fe3O4 nanoparticles.Methods The PEI-Fe3O4 nanoparticles were characterized by using transmission electron microscopy and dynamic light scattering.The primary cultured hAECs were labeled with the nanoparticles,and the labeling efficiency was evaluated by Prussian blue staining.The cell survival rate and viability were tested by using placenta blue staining and CCK-8 assay,respectively.Results The PEI-Fe3O4 nanoparticles were compact spheres with an average particle size of 13 nm,a hydrodynamic radius of 17.56 nm,and a zeta potential of + 34.5
PEI-Fe3O4纳米颗粒标记人羊膜上皮细胞
