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几种弓形虫ELISA试剂盒初步检测结果比较

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几种弓形虫ELISA试剂盒初步检测结果比较

牛安欧;冯友仁;刘文琦

【期刊名称】《中国人兽共患病学报》 【年(卷),期】2000(016)004

【摘要】本文选用5种国内弓形虫ELISA试剂盒检测已知同样样品,并且用国外试剂盒核查由该5种试剂盒提供的阳性样品,作试剂盒质量比较。结果 IgG-ELISA试剂A、B盒阳性检出率(敏感性)均为60%,A、B盒之间的符合率仅为50%,其特异性分别为35%和90%;C、D、E阳性检出率(敏感性)分别为30%、60%、70%,特异性分别为75%、54%、87%。核查A、B、D、E盒提供的样品结果:IgG符合率较好,均在90%以上。IgM符合率均为60%。鉴于目前国内ELISA试剂盒的现状,本文分析后建议,除改进质量外,宜选择多种试剂盒联合应用,避免漏检和误诊。临床诊断孕妇弓形虫病应谨慎从事。%Aim Five types of civil Toxoplasma gondii ELISA test kit were used to the detected specimen which proved to be the same, foreign-made test kit was used to examine the positive specimen given by these five types of test kit, as to make comparison about quality of test kit. Results IgG-ELISA A,B kits positive showing rates (sensitive)are all 60% ,the corresponding rate between A&B is merely 50% ,the specifics are 35%&90%. C, D,E kits' positive showing rates (sensitive)are 30 %, 60 %, 70 % the specifice are 75 %, 54 %, 87 %. The result about examining the probes given by A, B, D, E kit :IgG corresponding rates are better, which are all more than 90% ;IgM corresponding rates are all more than 60%.

It turn out that, according to the civil conditions of ELISA test kit, we suggest that besides the quality being improved in, all kinds of test kit should be used combinatively, so as to avoid neglect and mistake on examination, and we should be cautious in examining. 【总页数】3页(56-58)

【关键词】弓形虫 ELISA 试剂盒 【作者】牛安欧;冯友仁;刘文琦

【作者单位】同济医科大学寄生虫学教研室武汉,430030;同济医科大学寄生虫学教研室武汉,430030;同济医科大学寄生虫学教研室武汉,430030 【正文语种】中文 【中图分类】R382.5 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-zoonoses_thesis/0201231920801.html 【相关文献】

1.几种检测猪弓形虫病ELISA诊断试剂盒的对比 [J], 何勇; 朱艳平; 袁子国; 尹创成; 周东辉; 林瑞庆; 朱兴全; 翁亚彪

2.三种检测猪弓形虫病ELISA试剂盒的比较分析 [J], 黄翠琴; 何茜; 郑新添 3.用弓形虫多功能免疫吸附凝集试验试剂盒检测孕妇弓形虫感染… [J], 牛安欧; Jani.,R

4.SPA-ELISA检测弓形虫抗体诊断试剂盒的研制 [J], 刘佩梅; 杨秀珍; 吴增强 5.国产与进口弓形虫IgG抗体酶免检测试剂盒检测结果比较 [J], 段绩辉; 庄世

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几种弓形虫ELISA试剂盒初步检测结果比较

几种弓形虫ELISA试剂盒初步检测结果比较牛安欧;冯友仁;刘文琦【期刊名称】《中国人兽共患病学报》【年(卷),期】2000(016)004【摘要】本文选用5种国内弓形虫ELISA试剂盒检测已知同样样品,并且用国外试剂盒核查由该5种试剂盒提供的阳性样品,作试剂盒质量比较。结果IgG-ELISA试剂A、B盒阳性检出率(敏感性)均为
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