A novel, minimally invasive rat model of normothermic cardiopulmonary bypass model without

A novel, minimally invasive rat model of normothermic cardiopulmonary bypass model

without blood priming

Zhu Yaobin;Liu Donghai;Li Xiaofeng;Liu Aijun;Wang Qiang;Qiao Chenhui;Zhang Jing

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2014(127)008

【摘要】Background Cardiopulmonary bypass (CPB) has been shown to be associated with systemic inflammatory response leading to postoperative mechanisms

organ and

dysfunction.Elucidating

protective

the

underlying for

the

developing strategies

pathophysiological consequences of CPB have been hampered due to the absence of a satisfactory recovery animal model.The purpose of this study was to establish a novel,minimally invasive rat model of normothermic CPB model without blood priming.Methods Twenty adult male Sprague-Dawley rats weighing 450-560 g were randomly divided into CPB group (n=10) and control group (n=10).All rats were anaesthetized and mechanically ventilated.The carotid artery and jugular vein were cannulated.The blood was drained from the right atrium via the right jugular and further transferred by a miniaturized roller pump to a hollow fiber oxygenator and back to the rat via the left carotid artery.The volume of the priming solution,composed of 6％ HES130/0.4 and 125 IU heparin,was less than 12 ml.The surface of the