CUMS抑郁模型大鼠脑组织DA、5-HT及其代谢产物的浓
度变化
唐亚梅;陈春梅;刘勇
【期刊名称】《湖南师范大学学报（医学版）》 【年(卷),期】2012(009)003
【摘要】Objective We explored the related neurobiochemistry pathomechanism by the comparison of concentration of dopamine(DA), 5-hydroxytryptamine (5-HT) and their metabolites in brain tissue in the Chronic unpredicted mild stress (CUMS)depression model rats. Methods 24 male Spragur-Dawley (SD) rats were randomly assigned to 3 groups: control group, model group and fluoxetine group. CUMS with solitary condition was taken to manufacture depression rat model. After separated the brains tissues of CUMS depression rats, the levels of DA, 5-HT and their metabolites of the tissue homogenate from the medial prefrontal cortex (mPFC) and hippocampus were examined with Coularray Electrochemic detection for high performance liquid chromatogram (HPLC) technique. Results Compared to contronl group, the levels of DA, homovanillic acid (HVA), 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in the mPFC of depression model rats have significantly decreased(P<0.01 or 0.05), and the levels of DA, HVA and 5-HT in hippocampus have significantly decreased (P<0.01 or 0.05); Compared to model group, the levels of DA, HVA and 5 -HT in the
CUMS抑郁模型大鼠脑组织DA、5-HT及其代谢产物的浓度变化
