Product Data?SheetCinobufotalinCat. No.:CAS No.:分?式:分?量:作?靶点:作?通路:储存?式:HY-N08801108-68-5C??H??O?458.54OthersOthersPowderIn solvent-20°C4°C-80°C-20°C3 years2 years6 months1 monthInhibitors?Agonists?Screening Libraries溶解性数据体外实验DMSO : ≥ 35 mg/mL (76.33 mM)* \Mass1 mg5 mg10 mgSolventConcentration制备储备液1 mM5 mM10 mM2.1808 mL0.4362 mL0.2181 mL10.9042 mL2.1808 mL1.0904 mL21.8083 mL4.3617 mL2.1808 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；?旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存?式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个?内使?，-20°C 储存时，请在 1 个?内使?。体内实验请根据您的实验动物和给药?式选择适当的溶解?案。以下溶解?案都请先按照 In Vitro ?式配制澄清的储备液，再依次添加助溶剂： 为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的?作液，建议您现?现配，当天使?； 以下溶剂前显?的百分?是指该溶剂在您配制终溶液中的体积占?；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的?式助溶1. 请依序添加每种溶剂：?10% DMSO ?? 40% PEG300 ?? 5% Tween-80 ?? 45% salineSolubility: ≥ 2.17 mg/mL (4.73 mM); Clear solution此?案可获得 ≥ 2.17 mg/mL (4.73 mM，饱和度未知) 的澄清溶液。 以 1 mL ?作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加? 50 μL Tween-80，混合均匀；然后继续加? 450 μL ?理盐?定容? 1 mL。2. 请依序添加每种溶剂：?10% DMSO ?? 90% (20% SBE-β-CD in saline)Solubility: ≥ 2.17 mg/mL (4.73 mM); Clear solutionPage 1 of 2 www.MedChemExpress.cn
此?案可获得 ≥ 2.17 mg/mL (4.73 mM，饱和度未知) 的澄清溶液。 以 1 mL ?作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD ?理盐??溶液中，混合均匀。3. 请依序添加每种溶剂：?10% DMSO ?? 90% corn oilSolubility: ≥ 2.17 mg/mL (4.73 mM); Clear solution此?案可获得 ≥ 2.17 mg/mL (4.73 mM，饱和度未知) 的澄清溶液，此?案不适?于实验周期在半个?以上的实验。 以 1 mL ?作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL ??油中，混合均匀。BIOLOGICAL ACTIVITY?物活性Cinobufotalin 是强?类固醇或丁?烯内酯，是从蟾蜍的?肤分泌物中提取的。Cinobufotalin 已被?作强?药，利尿药和??药，也是潜在的抗肺癌药物。体外研究Cinobufotalin (0.1-10 μM; 72 hours; A549, H460 and HTB-58 human lung cancer cells) treatment induces cytotoxic effect against lung cancer cells[1].Cell Cytotoxicity Assay[1]Cell Line:Concentration:Incubation Time:Result:A549, H460 and HTB-58 human lung cancer cells0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM72 hoursSignificantly induced cell death in a concentration-dependent manner.体内研究Cinobufotalin (1-5 mg/kg; intraperitoneal injection; twice daily; for 1 weeks; male nude mice) treatment inhibits A549 lung cancer cell growth in vivo[1].Animal Model:Dosage:Administration:Result:Male nude mice (4-6 weeks old, BALB/c) with A549 cells[1]1 mg/kg or 5 mg/kgIntraperitoneal injection; twice daily; for 1 weeksInhibited A549 lung cancer cell growth in vivo.REFERENCES[1].?Kai S, et al. Pre-clinical evaluation of cinobufotalin as a potential anti-lung cancer agent. Biochem Biophys Res Commun. 2014 Sep 26;452(3):768-74.McePdfHeightCaution: Product has not been fully validated for medical applications. For research use only.
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