MLAA-22基因上游调控区的分离及启动子活性分析
崔鹤;张王刚
【期刊名称】《现代肿瘤医学》 【年(卷),期】2018(026)004
【摘要】目的:分离MLAA-22基因上游转录调控区序列,进行启动子活性鉴定.方法:人工合成MLAA-22基因上游转录调控区序列MLAA-22(-999 ～+1)和MLAA-22(-1999～+1)两个区段,将两个区段插入到双荧光素酶报告基因表达载体psiCHECK-2的BglⅡ/NheⅠ限制性内切酶位点中,构建双荧光素酶报告基因表达质粒,将重组质粒分别命名为psiCHECK-2-MLAA-22(-999 ～+1)和psiCHECK-2-MLAA-22(-1999 ～+1);采用双荧光素酶报告基因系统检测所分离区段的启动子活性.结果:MLAA-22(-999 ～+1)和MLAA-22(-1999～+1)两个区段均具有较强的相对荧光素酶活性,并且MLAA-22(-999～+1)区段的相对荧光素酶活性明显高于MLAA-22(-1999～+1)区段.结论:MLAA-22基因上游转录调控区(-999～+1)区段将是今后我们进行MLAA-22基因上游转录调控区启动子活性研究的重点区域.%Objective:To isolate the upstream transcriptional regulatory region of MLAA-22 gene,identify promoter activities of the isolated regions.Methods:-999 ～ + 1 and-1999 ～ + 1 of MLAA gene's upstream transcriptional regulatory region were synthesized and inserted into BglⅡ and NheⅠ restriction sites of dual-Luciferase reporter gene vector psiCHECK to construct recombinant plasmids.The recombinant plasmids were named psiCHECK-2-MLAA-22(-999 ～ + 1) and psiCHECK-2-MLAA-22(-1999～ + 1) respectively.The
MLAA-22基因上游调控区的分离及启动子活性分析
