免疫抑制剂和辛伐他汀对血管内皮细胞增殖及其分泌内皮
素的影响
张上珠;李梦涛;何建国;曾小峰
【期刊名称】《中华内科杂志》 【年(卷),期】2008(047)006
【摘要】Objective To evaluate the effects of four agents including cyclophosphomide,thalidomide,total glucosides of peony (TGP) and simvastatin on cell-proliferation and endothlin-1 secretion of human endothelial cells (ECs).Methods EA.by926 cells were cultured until confluence WSS achieved,then incubated separately for 24 h.48 h and 72 h with various concentration of these four agents.The proliferation of ECs waft detected with 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT).The amount of endothlin-1 in supernatants of ECs was determined with enzyme linked immunosorbent assay.Results (1)Cyclophosphomide,TGP and simvastatin inhibited ECs proliferation in a dose dependent manner at 72 h (P＜0.05).(2)Cyclophosphomide (50-2000 μmol/L) and simvastatin (5-10 μmol/L) decreased endothlin-1 secretion but not in dose dependent manner. Conclusion Cyclophosphomide.TGP and simvastatin can inhibit ECs proliferation.Cyelophosphomide and simvastatin can decrease endothlin-1 secretion of human ECs.%目的 研究环磷酰胺、沙立度胺、白芍总苷、辛伐他汀对体外培养的血管内皮细胞(EC)增殖及其分泌内皮素(ET)-1的
免疫抑制剂和辛伐他汀对血管内皮细胞增殖及其分泌内皮素的影响
