脂多糖对不同脑成熟程度早期新生鼠髓鞘发育的影响及肿瘤坏死因子

脂多糖对不同脑成熟程度早期新生鼠髓鞘发育的影响及肿

瘤坏死因子-α表达变化

安晶慧;毛健;周卓;高红

【期刊名称】《中国小儿急救医学》 【年(卷),期】2013(020)003

【摘要】Objective To monitor the function of infection on myelination in white matter damage,neonatal Wistar rats of postnatal day 2 (P2) and postnatal day 7 (P7) were injected intraperitoneally with the same doses of

lipopolysaccharides

(LPS),and

2',3

'-cyclic

nucleotide

phosphodiesterase (CNPase) and myelin basic protein (MBP) were labeled in immature oligodendrocytes and mature oligodendrocytes.To investigate the function of tumor necrosis factor(TNF)-α according to test the change of TNF-α expression in the brain.Methods Ninty-six neonatal Wistar rats were randomly divided into four groups (each group 24 rats):group A:LPS (5.0 mg/kg) was injected intraperitoneally on P2 ; group B:LPS (5.0 mg/kg) was injected intraperitoneally on P7 ;group C1 and C2 were control groups in which equal amount of normal saline was injected intraperitoneally on P2 or P7.The expression of CNPase at 24 h after injection and MBP at P14 in brain tissue of each group were measured by immunohistochemistry and express of TNF-α mRNA at 4 h after the injection was measured by RT-PCR.Results Punctate hemorrhage in the corpus callosum,external capsule and intraventricular