17β-雌二醇对大鼠肝移植缺血再灌注损伤的保护作用
杨小华;胡明政;秦磊;钱海鑫
【期刊名称】《中华实验外科杂志》 【年(卷),期】2011(028)012
【摘要】目的 观察17β-雌二醇对大鼠肝移植缺血再灌注损伤的保护作用.方法 将36只健康雄性SD大鼠随机分成3组:假手术组(Sham组)、缺血再灌注组(I/R组)、缺血再灌注+雌二醇组(I/R+ E2组),I/R组与I/R+ E2组改良二袖套法行肝移植术.术后6h处死受体,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)及丙二醛(MDA)含量,电镜下观察肝组织的超微结构,检测肝组织肿瘤坏死因子-α (TNF-α)的表达.结果 I/R组、I/R+ E2组ALT( 950.83±65.20)、(271.67±22.29) U/L,AST(2436.67±89.70)、(1152.83±85.37) U/L,MDA (0.63±0.03)、(0.40±0.02)nmol/mg,较Sham组(60.32±7.31) U/L、(38.12±4.33) U/L、(0.30±0.04) nmol/mg高(P＜0.05),I/R组均高于I/R+ E2组(P＜0.05).TNF-α在I/R组的表达最明显,I/R+ E2组的阳性表达少于I/R组.电镜下I/R组的肝细胞损伤显著重于I/R+ E2组.结论 雌激素对大鼠肝移植缺血再灌注损伤有保护作用,其机制可能与雌激素降低肝脏I/R时TNF-α和MDA有关.%Objective To observe the effect of 17β-estradiol (E2) on ischemia-reperfusion
injury
(IRI)
in
rat
orthotopic
liver
transplantation.Methods Thirty-six SD male rats were randomized into three groups:sham group,I/R group,I/R + E2 group.The rate in I/R group and I/R + E2 group were subjected to liver transplantation by modified two-cuffed methods.Six h after reperfusion,the recipients were
17β-雌二醇对大鼠肝移植缺血再灌注损伤的保护作用
