Analytical characterization and structure elucidation of metabolites from Aspergillus ochr

Analytical characterization and structure elucidation of metabolites from Aspergillus

ochraceus MP2 fungi

Meenupriya J;Thangaraj M

【期刊名称】《亚太热带生物医学杂志（英文版）》 【年(卷),期】2011(000)005

【摘要】Objective: To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus (A. ochraceus) MP2 fungi. Methods: The anti bacterial activity of marine sponge derived fungi A. ochraceus MP2 was thoroughly investigated against antagonistic human pathogens. The optimum inhibitory concentration of the fungi in the elite solvent was also determined. The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis. The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer (ITS) region of 5.8s rRNA using specific ITS primer. The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK. Results: Three bioactive compounds were characterized to reveal their identity, chemical formula and structure. The first elutant was identified as α-Campholene aldehyde with chemical formula C10