兔脊髓缺血再灌注损伤后能量代谢与NF-κBp65表达变化
章建平;方华;张竞超;章放香
【期刊名称】《中国医学创新》 【年(卷),期】2015(000)009
【摘要】目的：探讨脊髓缺血再灌注损伤（SCIRI）中能量代谢与NF-κBp65的变化规律。方法：40只新西兰大白兔采用肾下腹主动脉阻断45 min，建立脊髓缺血再灌注损伤模型。分别于缺血前、缺血45 min及再灌注30、60、120 min时取脊髓测定腺苷酸（ADP、AMP、ATP）、活性氧（ROS）、核转录因子-κBp65（NF-κBp65）、抑制蛋白-κBα（I-κBα）和细胞间黏附分子-1（ICAM-1）蛋白表达变化。结果：与缺血前比较，缺血45 min及再灌注30、60、120 min时脊髓腺苷酸及I-κBα均降低（P<0.01），ROS、NF-κBp65和ICAM-1均升高（P<0.01）。结论：SCIRI中ICAM-1和NF-κBp65表达上调加重脊髓能量代谢障碍。%Objective:To assess the changes of energy metabolism and NF-κBp65 during spinal cord ischemia and reperfusion injury(SCIRI). Method:The SCIRI model was made by clamping the infrarenal aortic for 45 minutes in 40 rabbits.Reactive oxygen species(ROS),the
expression
of nuclear
factor-kappa
Bp65(NF-
κBp65),inhibitor-Kappa Bα(I-κBα),intercellular adhesion molecule(ICAM-1)and the content of adenine nucleotide(ADP,AMP and ATP)in spinal cord tissue were determined before ischemia,45 minutes after ischemia,30,60 minutes and 120 minutes after reperfusion. Result:The spinal cord content of adenine nucleotide and I-κBαdecreased while
兔脊髓缺血再灌注损伤后能量代谢与NF-κBp65表达变化
