多重耐药鲍曼不动杆菌定植与感染表达差异的分析
隋文君;王玫;黄艳飞;鲁辛辛
【期刊名称】《中华医学杂志》 【年(卷),期】2016(096)041
【摘要】Objective To analyze the protein expression differences between colonized multidrug-resistant acinetobacter baumannii (MDR-AB) and infectious MDR-AB using comparative proteomics and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and to explore the molecular characteristics of MDR-AB infections.Methods A total of 59 MDR-AB strains including 23 of bloodstream infections MDR-AB and 36 of nasal,pharyngeal or environmental colonization MDR-AB were collected from Beijing Tongren Hospital between January 2008 and December 2011.MALDI-TOF MS cluster analysis was applied to identify the differences of protein expression between infection strains and colonization strains (including patient colonization and environmental colonization) of MDR-AB.Two-dimensional protein differences electrophoresis and MALDI-TOF-TOF MS were used to analyze the protein expression differences between bloodstream infection strain and nasal colonization strain isolated from the same patient.Results ST92 was the main multiple locus sequence typing of 23 bloodstream infections MDR-AB.Cluster analysis showed that there were differences of protein
多重耐药鲍曼不动杆菌定植与感染表达差异的分析
