秋水仙素诱导白皮松多倍体的研究
闫辉;张利;于淑惠;左曼曼;樊金会
【期刊名称】《山东农业大学学报（自然科学版）》 【年(卷),期】2012(043)002
【摘要】Cotyledon explants of mature embryos of Pinus bungeana tissue were culture, the use of colchine induced polyploid. Cotyledons were cultured for 10 days, with the quality of volume fraction of 0. 01%, 0. 03%, 0. 05% of colchicine immersion, respectively 6 h, 12 h, 24 h, observation oi cotyledon growth and bud differentiation status, identification of chromosome doubling in 20 days. Results indicated that: different concentrations of colchicine effectfed cotyledon growth effect. Concertrstian was higher, processing time was longer, the higher the death; colchicine treated shoci regeneration is slow, and snoot regeneration rale increased with the concentration decreased; colchicine treated cotyledons chromosomes nave doubled, but with colchicine concentration enhancement and treatment the extension of time, polyploid induction rate showed a downward trend. Data displayed quality score of 0. 03% for the combination of 12 h treated with colchicine, polyploid inJuction rate was highest, 40. 5%. The study on polyploid induction of Pinus bungeann system were initially established .%以白皮松成熟胚的子叶作为外植体进行组织培养,利用秋水仙素诱导多倍体.将子叶预培养10 d后,用质量体积分数为0.01％、0.03％、0.05％
秋水仙素诱导白皮松多倍体的研究
