非小细胞肺癌EGFR、KRAS基因突变与RRM1、
TUBB3mRNA表达水平的相关性
任保瑞;朱彦君;王桂洪;刘颖汇
【期刊名称】《现代肿瘤医学》 【年(卷),期】2016(024)014
【摘要】Objective:To investigate whether EGFR or KRAS gene mutations are correlated with the mRNA ex-pression levels of ribonucleotide reductase subunit M1(RRM1)and class III β - tubulin(TUBB3)in non - small cell lung cancer(NSCLC),and their clinical significance. Methods:A total of 69 NSCLC tissues were from Thoracic Surgery of Air Force General Hospital from June 2010 to October 2014,and were obtained by intraoperative cut. EGFR and KRAS mutations were detected with xTAG liquidchip technology(xTAG - LCT),and mRNA expression levels of RRM1 and TUBB3 genes were detected by branched DNA - liquidchip technology(bDNA - LCT). Results:Of 69 cases NSCLC tissue,28 cases were positive for EGFR mutations(40. 6% ,28 / 69),13 cases were positive for KRAS mutations(18. 8% ,13 / 69). EGFR gene mutations were relevant to gender( P = 0 . 005 ),pathological type(P= 0. 036),smoking(P = 0. 029). KRAS gene mutations were related to gender(P = 0. 007). The mRNA expression levels of TUBB3 genes were related to the pathological type(P = 0. 008). The mRNA expression levels of RRM1 gene were not relevant to clinical and phathological characteristics of
非小细胞肺癌EGFR、KRAS基因突变与RRM1、TUBB3mRNA表达水平的相关性
