Development and identification of glyphosate-tolerant transgenic soybean via direct select

Development and identification of glyphosate-tolerant transgenic soybean via direct selection

with glyphosate

GUO Bing-fu; HONG Hui-long; HAN Jia-nan; ZHANG Li-juan; LIU Zhang-xiong; GUO Yong; QIU Li-juan

【期刊名称】《农业科学学报：英文版》 【年(卷),期】2024(019)005

【摘要】Glyphosate-tolerant soybean is the most widely planted genetically modified crop worldwide. However, soybean remains recalcitrant to routine transformation because of the low infection efficiency of Agrobacterium to soybean and lack of useful selectable markers. In this study, several Agrobacterium strains and cell densities were compared by transient expression of the GUS gene. The results showed that Agrobacterium strain Ag10 at cell densities of OD_(600) of 0.6–0.9 yielded the highest infection efficiency in Agrobacterium-mediated

soybean

cotyledonary

node

transformation

system.

Meanwhile, a simple and rapid method was developed for identification of glyphosate tolerance in putative T_0 transgenic plants, consisting of spotting plantlets with 1 μL Roundup~?. The whole cycle of genetic transformation could be shortened to about 3 mon by highly efficient selection with glyphosate during the transformation process and application of the spot assay in putative T_0 transgenic plantlets. The

transformation frequency ranged from 2.9 to 5.6%. This study provides an improved protocol for development and identification of glyphosate-tolerant transgenic soybeans. 【总页数】11页(P.1186-1196)

【关键词】soybean; transformation; spot assay; glyphosate selection; transgenic plants

【作者】GUO Bing-fu; HONG Hui-long; HAN Jia-nan; ZHANG Li-juan; LIU Zhang-xiong; GUO Yong; QIU Li-juan

【作者单位】National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/MOA Key Lab of Soybean Biology/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.ChinaJiangxi Province Key Laboratory of Oilcrops Biology/Crops Research Institute Jiangxi Academy of Agricultural Sciences Nanchang 330200 P.R.China; National Key Facility for Crop Gene Resources and Genetic

Improvement(NFCRI)/MOA

Key

Lab

of

Soybean

Biology/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China 【正文语种】中文 【中图分类】R73 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_journal-agricultural-sciences-english_thesis/0201278826602.html

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