Effect of interleukin-2 on neurogliocyte and neuron apoptosis in rat models of acute spina

Effect of interleukin-2 on neurogliocyte and neuron apoptosis in rat models of acute spinal cord injury

Chuanli Zhou;Xiaoliang Chen;Ying Wang

【期刊名称】《中国神经再生研究（英文版）》 【年(卷),期】2008(003)011

【摘要】BACKGROUND: Interleukin-2 (IL-2) may influence the growth and survival of nerve cells following spinal cord injury and resuscitate the proliferation and maturation of oligodendrocytes. OBJECTIVE: To observe the effect of IL-2 on neuronal apoptosis of neurogliocytes at different times following acute spinal cord injury in rats. DESIGN, TIME AND SETTING: A randomized grouping trial based on cellular morphology was performed at the Institute of Traumatic Orthopedics of Shandong Province between October 2004 and January 2006. MATERIALS: A total of 72 adult, male, Sprague Dawley rats were included in this study and were divided into a control group and an IL-2 group. The Bcl-2 monoclonal antibody and TUNEL kit were purchased from Wunan Boster Biological Technology Corporation. METHODS: Spinal cord injury was induced in all the rats by dropping a weight from a height of 25 cm onto the exposed spinal cord at vertebral levels T7-11, thus producing a mild lesion. Immediately following the modeling, the rats were injected with daily IL-2 (10 μL) intramuscularly (the IL-2 group). Other rats received an injection of physiological saline 0.5 mL/d (the