Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and T

Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their

Parents

Zhu-Qing REN;Yuan-Zhu XIONG;Chang-Yan DENG;Ming-Gang LEI

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2006(038)011

【摘要】In order to investigate porcine heterosis on the molecular basis, Large White (L), a European purebred, and Meishan (M), a Chinese indigenous purebred, were hybridized directly and reciprocally to produce F1 hybrids, Large White×Meishan (LM) and Meishan×Large White (ML) pigs. Using mRNA differential display, we found an expression sequence tag (EST) differentially expressed in F1 hybrids and their parents, designated as EST55, which was homologous to human and murine skeletal muscle protein (SMPX), and the full-length cDNA of porcine SMPX was cloned by the rapid amplification of cDNA end (RACE) method. Translation of the mRNA transcript revealed an open reading frame (ORF) of 86 amino acid residues encoding a nuclear location signal peptide, two overlapping casein kinase Ⅱ phosphorylation sites and one N-glycosylation site with theoretical molecular weight of 9.3 kDa. Alignment analysis revealed that the deduced protein sequence shared 94%, 83% and 78% homology with that of its human, mouse and rat counterparts, respectively. Reverse transcription-polymerase chain