Sepsis Strengthens Antagonistic Actions of Neostigmine on Rocuronium in a Rat Model of Cec

Sepsis Strengthens Antagonistic Actions of Neostigmine on Rocuronium in a Rat Model of

Cecal Ligation and Puncture

Jin Wu;Tian Jin;Hong Wang;Shi-Tong Li

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2016(129)012

【摘要】Background:The antagonistic actions of anticholinesterase drugs on non-depolarizing muscle relaxants are theoretically related to the activity ofacetylcholinesterase (AChE) in the neuromuscular junction (NMJ).However,till date the changes of AChE activity in the NMJ during sepsis have not been directly investigated.We aimed to investigate the effects of sepsis on the antagonistic actions ofneostigmine on rocuronium (Roc) and the underlying changes of AChE activity in the NMJ in a rat model of cecal ligation and puncture (CLP).Methods:A total of 28 male adult Sprague-Dawley rats were randomized to undergo a sham surgery (the sham group,n =12) or CLP (the septic group,n =16).After 24 h,the time-response curves of the antagonistic actions of 0.1 or 0.5 μmol/L of neostigmine on Roc (10 μmol/L)-depressed diaphragm twitch tension were measured.Meanwhile,the activity of AChE in the NMJ was detected using a modified Karnovsky and Roots method.The mRNA levels of the primary transcript and the type T transcript of AChE (AChET) in the diaphragm were determined by real-