LncRNA UCA1对卵巢癌细胞侵袭及迁移的影响及机制
王帆;周戬平;谢小娟;胡军;郭华;胡巧侠
【期刊名称】《现代肿瘤医学》 【年(卷),期】2015(000)001
【摘要】Objective:To study the invasion and migration ability of long chain non-coding RNA UCA1 in ovari-an cancer cells SKOV3. Methods:Constructing eukaryotic expression vector pcDNA/UCA1. pcDNA/UCA1 construct was transfected into SKOV3 cells by lipofectamine2000 for 24h and selected with 500μg/ml G418 for 3 weeks,trans-fected with pcDNA3. 1 empty vector acted as a control. The positive clone was identified by reverse transcription poly-merase chain reaction( RT -PCR ) for UCA 1 RNA expression . Cell invasion and migration ability was assessed by using millicell chamber. MMP2 and MMP9 protein level was detected by Western blotting analysis. Results:We successfully established the SKOV3 cell stable expressing pcDNA/UCA1 transfectant. Exogenous expression of UCA1 in SKOV3 cells enhanced migration and invasion ability of cells. Conclusion:MMP2 and MMP9 protein expression in-creased in SKOV3/pcDNA-UCA1 cells compared with SKOV3/pcDNA3. 1 cells. Which suggested that UCA1 RNA might play some role in SKOV3 cells invasion and progression.%目的：探讨长链非编码RNA UCA1对卵巢癌细胞SKOV3侵袭及迁移能力的影响及可能的作用机制。方法：用脂质体2000将pcDNA/UCA1表达载体及
LncRNA UCA1对卵巢癌细胞侵袭及迁移的影响及机制
