细胞培养PBS配方

Phosphate-BufferedSaline (PBS; 1×, pH 7.4)

Reagent

Final concentration

Quantity (g)

(mM)

8.0 0.20 1.14 （2.874） 0.20 137.0 2.7 8.0 1.5 NaCl KCl Na2HPO4（12H2O） KH2PO4 Combine all ingredients in ～800 mL of H2O and stir to dissolve. Adjust pH to 7.4 and bring final volume to 1 L. Sterilize by autoclaving. The most common composition of PBS (1X) Salt Concentration (mmol/L) Concentration (g/L) NaCl 137 8.0 KCl 2.7 0.2 Na2HPO4 10 1.44 KH2PO4 1.8 0.24 Start with 800 mL of distilled water to dissolve all salts. Adjust the pH to 7.4 with HCl. Add distilled water to a total volume of 1 liter. The resultant 1x PBS should have a final concentration of 10 mM PO43-, 137 mMNaCl, and 2.7 mMKCl.