西地那非抑制高肺血流肺动脉高压大鼠肺血管重构和上调
肺血管Kv1.5mRNA表达
胡蕾;谈林华;林隆;何小军;范佳杰
【期刊名称】《中华小儿外科杂志》 【年(卷),期】2010(031)007
【摘要】Objective To investigate the effects of sildenafil on pulmonary vascular remodeling and voltage-gated potassium channeI Kv1.5 mRNA expression of pulmonary vasculature in secondary pulmonary hypertension due to left-to-right shunt in rats.Methods Male twenty-seven SD rats were randomly divided into sham group(n=9),shunt group(n=9)and shunt+sildenafil group(n=9).A left-to-right shunt was established on shunt group and shunt+sildenafil group rats by making fistula between abdominal aorta and inferior vena cava.Rats of the shunt+sildenafil group were administered sildenafil by gastric gavage at a dose of 10 mg·kg-1·d-1;whereas the rats of the sham group and the shunt group were fed with the same amount of saline. Eleven weeks later,mean pulmonary artery pressure(mPAP)and systolic pulmonary artery pressure(sPAP)were measured.The ratio of right ventricular mass to left ventricular plus septal mass[Rv/(LV+S)]was alao calculated and taken as a marker of the severity of right ventricular hypertrophy.The pathological changes of pulmonary vasculature were evaluated by calculating the relative medial thickness(RMT) of middle and small
西地那非抑制高肺血流肺动脉高压大鼠肺血管重构和上调肺血管Kv1.5mRNA表达
