Prevalence of Helicobacter pylori cagA genotype among dyspeptic patients in Southern Thailand

Prevalence of Helicobacter pylori cagA genotype among dyspeptic patients in Southern Thailand

Sueptrakool Wisessombat;Chatruthai Meethai

【期刊名称】《海岸生命医学杂志（英文版）》 【年(卷),期】2014(000)011

【摘要】Objective: To investigate the prevalence of Helicobacter pylori (H. pylori) infection in dyspepsia patients and its relation to virulence factor cagA gene.
Methods: In total, 110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex polymerase chain reaction (PCR).
Results: Multiplex PCR detected three genes of 16S rRNA, cagA, and ureC. H. pylori was detected in 14 gastric biopsies (13%). Significantly higher numbers of female were infected. Furthermore,cag
A gene was found in all H. pylori-positive specimens. In addition, the result indicated that the multiplex PCR with annealing temperature at 57 oC was able to effectively amplify specific products.
Conclusions:The results confirmed high prevalence of cagA gene in H. pylori among dyspeptic patients in Southern Thailand. 【总页数】4页(895-898) 【关键词】

【作者】Sueptrakool Wisessombat;Chatruthai Meethai

【作者单位】School of Allied Health Sciences and Public Health, Walailak