a-SMA与Vimentin在慢性硬膜下血肿外膜中的表达及意
义
李曦;刘艳;徐建荣;陈书达;信照亮
【期刊名称】《浙江医学》 【年(卷),期】2011(033)009
【摘要】目的 采用a-平滑肌肌动蛋白(a-SMA)与波形蛋白 (VIM)鉴定和分类慢性硬膜下血肿(CSDH)外膜中的肌成纤维细胞 (MFB),并探讨两者表达的意义.方法 选取CSDH 9例患者的硬脑膜和其下的血肿膜,采用免疫组化法检测a-SMA和VIM在CSDH外膜中的表达,采用透射电镜观察细胞形态结构.结果 CSDH血肿膜中细胞数量较多,可见许多新生毛细血管;共同表达a-SMA和VIM的细胞有两种,其中一类细胞呈梭形(为肌成纤维细胞),另一类是血管管壁的细胞(为血管内皮细胞);阳性表达的棕黄色颗粒状均位于胞质内.电镜观察可见MFB细胞特征性形态,胞核有很多切迹,沿细胞长轴的边缘有束状肌动蛋白细丝排列.结论 a-SMA与VIM的广泛表达和持续分布可能在CSDH的发生发展中有重要作用.%Objective To explore the expression and significance of myofibroblasts (MFB) in chronic subdural hematoma (CSDH)of outer membranes
by
identifying
and
categorizing
a-
SMA
and
Vimentin.Methods The expressions of Alpha smooth muscleactin (a - SMA) and Vimentin in the chronic subdural hematoma of cerebral dura maters and outer membranes of 9 patients were detected by immunohistochemistry.Morphological change in outer membrane was observed with electromicroscope.Results There were plenty of cells and
newly reproductive capillaries in the outer membrane of CSDH.a- SMA and Vimentin were co- expressed in two cells, one was MFB with a spindle- shaped, the other was vascular endothelial cell.Positive staining were expressed in the cytoplasm.Marker of MFB was observed electron microscope.There were much notched nucleus and bundle of microfilaments running parallel to the long axis through the cytoplasm.Conclusion MFB were persistently distributed in the chronic subdural hematoma (CSDH)of outer membranes, which played an essentialrole in the development of CSDH. 【总页数】3页(1272-1274)
【关键词】a-SMA;Vimentin;慢性硬膜下血肿;肌成纤维细胞 【作者】李曦;刘艳;徐建荣;陈书达;信照亮
【作者单位】325035,温州医学院;325035,温州医学院;义乌市中心医院;浙江省人民医院;浙江医学高等专科学校 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_zhejiang-medical-journal_thesis/0201255020590.html 【相关文献】
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a-SMA与Vimentin在慢性硬膜下血肿外膜中的表达及意义
