The Effect of Peony and Licorice Decoction on the Voltage-Gated Sodium Channel Subtype 1.4

The Effect of Peony and Licorice Decoction on the Voltage-Gated Sodium Channel Subtype 1.4 Based

on Standard Decoction

Guang-Wei Zhu;Jie Guo;Ya-Jing Li;Lu Luo;Toru Sugita;Takehisa Tomoda;;;;;;;;;;;;;;;;;;;

【期刊名称】《世界中医药杂志：英文版》 【年(卷),期】2018(000)002

【摘要】Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components

on

the

Nav1.4

voltage-gated

sodium

channels

（VGSCs）.Materials and Methods:Writhing test was carried out with ICR mice.Paeonia lactiflora and Glycyrrhiza uralensis group were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 2.94 mg/ml,1.47 mg/ml,and 0.74 mg/ml using intragastric administration,respectively.Peony and licorice decoction groups were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 5.89 mg/ml,2.94 mg/ml,and 1.47 mg/ml using intragastric administration,respectively.For electrophysiology studies,each freeze-dried powder was dissolved in DMSO to make 10 mg/ml and 50 mg/ml stock solutions.The electrophysiological recordings were obtained under visual control of a microscope.For UPLC analysis,the freeze-dried powder was dissolved in methanol and then determines the contents of