伴有JAK2V617F突变的慢性中性粒细胞白血病1例并文
献复习
张男;庄倩;王韫秀;刘璇;张继红
【期刊名称】《现代肿瘤医学》 【年(卷),期】2012(020)005
【摘要】目的:探讨伴有JAK2基因V617F突变的慢性中性粒细胞白血病(CNL)的实验室特征.方法:通过骨髓涂片进行细胞形态学分析诊断；采用实时荧光定量PCR方法检测BCR-ABL融合基因；实时荧光定量PCR方法及DNA测序方法检测JAK2 V617F突变；RHG显带进行常规染色体核型分析.结果:细胞形态学诊断为慢性中性粒细胞白血病；BCR/ABL融合基因为阴性；存在JAK2 V617F杂合突变；染色体分析结果为正常核型.结论:JAK2 V617F突变在慢性中性粒细胞白血病中很少见,具有该突变的CNL患者有较长的生存期,JAK2 V617F突变可能提示预后良好.%Objective: To investigate the laboratory character of chronic neutrophilic leukemia (CNL) with an associated V617F JAK2 tyrosine kinase mutation. Methods: To diagnoze CNL by cell morphology,detect BCR - ABL fusion gene by real - time quantitative PCR,and V617F JAK2 tyrosine kinase mutation by real - time quantitative PCR and DNA sequencing;To analyze chromosome karyotype. Results: CNL was diagnosed by cell morphology. BCR -ABL fusion gene( negative) ;existence of V617F JAK2 tyrosine kinase mutation, normal karyotype were observed. Conclusion; V617F JAK2 tyrosine kinase mutation is rare in CNL,CNL with an associated V617F
伴有JAK2V617F突变的慢性中性粒细胞白血病1例并文献复习
