Product Data Sheet
Recombinant Mouse Macrophage Colony Stimulating Factor
Cat. No.:Synonyms:Species:Source:Accession:Gene ID:Molecular Weight:
HY-P7085rMuM-CSF; CSF-1; MGI-IMMouseE. coliP0714112977Approximately 30 kDa
Inhibitors?Agonists?Screening LibrariesPROPERTIES
AA Sequence
MKEVSEHCSHQEQLDDPVCYLQELSNNLNSFFNETKNLLE
MIGNGHLKVLLKKAFFLVQDCFTKDYEEQNKDWNIFTKNC
QQLIDSQMETIIDETMRFKDKACVRTFHETNNSFAKCSSR
SCQIAFEFVDNTPNANATERPLQLLEKIKNDVVTKP
Biological Activity
The ED50 is <3 ng/mL as measured by murine M-NFS-60 cells, corresponding to a specific activity of >3.3 × 105 units/mg.
AppearanceFormulationEndotoxin LevelReconsititution
Lyophilized powder.
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.<0.2 EU/μg, determined by LAL method.
Reconstitute the lyophilized recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF) to 100 μg/mL using ddH2O.
Storage & StabilityLyophilized recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
ShippingRoom temperature in continental US; may vary elsewhere.
DESCRIPTION
Background
Macrophage Colony Stimulating Factor (M-CSF) is a pro-inflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms- as a secreted glycoprotein, a cell-surface protein and a proteoglycan[1]. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage
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lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis[2].
REFERENCES
[1].?Hamilton JA. Colony-stimulating factors in inflammation and autoimmunity. Nat Rev Immunol. 2008 Jul;8(7):533-44.
[2].?Rioja I, et al. Potential novel biomarkers of disease activity in rheumatoid arthritis patients: CXCL13, CCL23, transforming growth factor alpha, tumor necrosis factor receptor superfamily member 9, and macrophage colony-stimulating factor. Arthritis Rheum. 2008 Aug;58(8):2257-67.
McePdfHeightCaution: Product has not been fully validated for medical applications. For research use only.
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重组小鼠巨噬细胞群刺激因子作用机制- Medchemexpress- MCE中国
