Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa
Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa
Zhishui He;Rong Xie;Yanzhang Wang;Huasong Zou;Jiabi Zhu;Guanqiao Yu
【期刊名称】《生物化学与生物物理学报:英文版》 【年(卷),期】2008(040)003
【摘要】Some members of the heat shock protein 70 (HSP70) family have important functions in organism development. Here, we identified an alfalfa (Medicago sativa L. cv. Algonquin) HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids.This protein contains all conserved domains,motifs.and characteristic sequences of plant HSP70s.The expression of MsHsP70-1 is enhanced in nodule compared withroot,stem,leaf,and flower,and throughout the process of nodule development.Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia.These results suggested that MsHSP70-1 might play an important role in alfalfa nodule development. 【总页数】8页(209-216)
【关键词】heat shock protein 70;cDNA array;symbiotic nitrogen fixation;nodule development
【作者】Zhishui He;Rong Xie;Yanzhang Wang;Huasong Zou;Jiabi Zhu;Guanqiao Yu
Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa
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