Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa

Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa

Zhishui He;Rong Xie;Yanzhang Wang;Huasong Zou;Jiabi Zhu;Guanqiao Yu

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2008(040)003

【摘要】Some members of the heat shock protein 70 (HSP70) family have important functions in organism development. Here, we identified an alfalfa (Medicago sativa L. cv. Algonquin) HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids.This protein contains all conserved domains,motifs.and characteristic sequences of plant HSP70s.The expression of MsHsP70-1 is enhanced in nodule compared withroot,stem,leaf,and flower,and throughout the process of nodule development.Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia.These results suggested that MsHSP70-1 might play an important role in alfalfa nodule development. 【总页数】8页(209-216)

【关键词】heat shock protein 70;cDNA array;symbiotic nitrogen fixation;nodule development

【作者】Zhishui He;Rong Xie;Yanzhang Wang;Huasong Zou;Jiabi Zhu;Guanqiao Yu