EFFECTS OF TOTAL SAPONINS OF PANAX NOTOGINSENG AND LIGUSTRAZINE ON THE PROLIFERATION OF CE

EFFECTS OF TOTAL SAPONINS OF PANAX NOTOGINSENG AND LIGUSTRAZINE ON THE PROLIFERATION OF CEREBRAL MICROVASCULAR

ENDOTHELIAL CELLS OF RATS

李敏杰;刘勇;丁海燕

【期刊名称】《西安医科大学学报(英文版)》 【年(卷),期】2002(014)002

【摘要】Objective To investigate the effects of Total Saponins of Panax notoginseng(PNS) and Liguastrazine(LIT) on the proliferation of cultured cerebral microvascular endothelial cells. Methods The inverted microscope was used to observe endothelial cells and immunochemical methods was also used to detect FVIII-related antigens so as to observe endothelial cells. PNS or LIT in concentrations 0.5 g*L-1, 1.0 g*L-1 and 2.0 g*L-1 were used on the cultured cerebral endothelial cells of rats for 24 hours. MTT method was adopted to determine the outcome of endothelial proliferation. Results 1. Immunochemical methods was used to detect FVIII-related antigens. The brownish yellow showed positive, and the observation of the cultured endothelial cells under inverted microscope showed that the cells appeared to be in the morphological form of cobble-stones. 2. PNS in lower concentration (0.5 g*L-1) could facilitate the proliferation of the cells, while 1 g*L-1 and 2 g*L-1 of PNS could inhibit the proliferation of the cells. 0.5 g*L-1 of LIT could