Methylation profiling of twenty four genes and the concordant methylation behaviours of ni

Methylation profiling of twenty four genes and the concordant methylation behaviours of nineteen genes that may contribute to hepatocellular

carcinogenesis

JIAN YU;HONG YU ZHANG;ZHEN ZHONG MA;WEI LU;YI FEI WANG;JINGDE ZHU

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2003(013)005

【摘要】To determine the possible role of the epigenetic mechanisms in carcinogenesis of the hepatocellular carcinoma,we methylation-profiled the promoter CpG islands of twenty four genes both in HCC tumors and the neighboring non-cancerous tissues of twenty eight patients using the methylation-specific PCR (MSP) method in conjunction with the DNA sequencing. In comparison with the normal liver tissues from the healthy donors, it was found that while remained unmethylated the ABL, CAV, EPO, GATA3, LKB 1, NEP, NFL, NIS and p27KIP1 genes, varying extents of the HCC specific hypermethylation were found associated with the ABO, AR, CSPG2, cyclin al, DBCCRl,GALR2, IRF7, MGMT, MT1A, MYOD1, OCT6, p57KIP2, p73, WT1 genes, and demethylation with the MAGEA1 gene, respectively. Judged by whether the hypermethylated occurred in HCC more frequently than in their neighboring normal tissues, the hypermethylation status of the AR, DBCCR1, IRF7, OCT6, and p73 genes was considered as the event specific to the late stage, while