Effects of plant extract neferine on cyclic adenosine monophosphate and cyclic guanosine m

Effects of plant extract neferine on cyclic adenosine monophosphate and cyclic guanosine

monophosphate levels in rabbit corpus

cavernosum in vitro

Jun Chen;Ji-Hong Liu;Tao Wang;Heng-Jun Xiao;Chun-Ping Yin;Jun Yang

【期刊名称】《亚洲男性学杂志（英文版）》 【年(卷),期】2008(010)002

【摘要】Aim: To further investigate the relaxation mechanism of neferine (Nef), a bis-benzylisoquinoline alkaloid extracted (isolated) from the green seed embryo of Nelumbo nucifera Gaertn in China, on rabbit corpus cavernosum tissue in vitro. Methods: The effects of Nef on the concentrations of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in isolated and incubated rabbit corpus cavernosum tissue were re-corded using125Ⅰ radioimmunoassay. Results: The basal concentration of cAMP in corpus cavernosum tissue was 5.67±0.97 pmol/mg. Nef increased the cAMP concentration in a dose-dependent manner (P < 0.05), but this effect was not inhibited by an adenylate cyclase inhibitor (cis-N-[2-phenylcyclopentyl]azacyclotridec-1-en-2-amine,MDL-12, 330A) (P > 0.05). The accumulation of cAMP induced by prostaglandin E1(PGE1, a stimulator of cAMP production) was also augmented by Nef in a dose-dependent manner (P < 0.05). The basal concentration of cGMP in