组蛋白去乙酰化酶抑制剂MS-275对乳腺癌MCF7细胞周
期作用及分子机制探讨
陈卫花;李龙飞;刘霞;程慧敏
【期刊名称】《徐州医学院学报》 【年(卷),期】2011(031)012
【摘要】Objective To study the effect of histonedeacetylase inhibitorMS-275 to the breast cancerMCF7 cell cycle and the mechanisn Methods 0.5, 1.0, 1.5 μmol/ LMS-275 affects the MCF7 cell for 24 hours, observes different density MS-275 witiMTT to theMCF7 cell s growth inhibitory action; flow cytanetry examination cell growth and the cycle; Western- blotting exanined MS-275 to themanmary gland cancer cell cycle related gene expression Results 1.0 moJ'L densityMS-275 had tie obvious growth inhibitory effect to theMCF7 cell and presented certain dose effect relationship, may induce the breast cancer cellMCF7 mitotic cycle above thel.O mol'L density tohinderobviously, may strengthen the p21, p27 gene obviously the expression, reduced CCND1, the CdM D gene expression obviously Conclusion Certain dosage\sMS-275 suppresses breast cancerMCF7 cell s growth, may through regulatemany mitotic cycle related gene induction manmary gland cancer cell mitotic cycle to hinder, the cell hinders occurrence and p21, p27 gene expression increase and CCNDl, Cdl4 D gene expression reduced related%目的 研究组蛋白去乙酰化酶抑制剂MS-275对乳腺癌
组蛋白去乙酰化酶抑制剂MS-275对乳腺癌MCF7细胞周期作用及分子机制探讨
