Nitric Oxide and Brassinosteroids Mediated Fungal Endophyte-Induced Volatile Oil Productio

Nitric Oxide and Brassinosteroids Mediated Fungal

Endophyte-Induced Volatile Oil Production Through Protein Phosphorylation Pathways in

Atractylodes lancea Plantlets

Cheng-Gang Ren;Chuan-Chao Dai

【期刊名称】《植物学报（英文版）》 【年(卷),期】2013(000)011

【摘要】Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide (NO) and brassinolide (BL) in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. AL12 enhanced the activities of total protein phosphorylation, Ca2t-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein