VEGF siRNA及bFGF siRNA对胰腺癌细胞增殖和侵袭性
的影响
闫长青;赵玉沛
【期刊名称】《中华外科杂志》 【年(卷),期】2010(048)008
【摘要】Objective To investigate the relationship between expression of angiogenic factors and invasion and proliferation of pancreatic cancer cell.Methods The three pancreatic cancer cell lines of SW1990,Panc-1 and PCT-3 were divided into four groups respectively:concrol group,VEGF siRNA group,bFGF siRNA group and VEGF siRNA + bFGF siRNA group.The expression and the secretion of VEGF and bFGF in the three cell lines were inhabited by VEGF siRNA and bFGF siRNA.The proliferation and the invasion of the three cell lines were determined by CCK-8 and Boyden Chamber invasion tests.Results Expressions of VEGF and bFGF in three cell lines were significantly inhibited by VEGF siRNA and bFGF siRNA.The proliferation was inhabited by VEGF siRNA and bFGF siRNA in SW1990 and Panc-1(P＜0.05),while wasn't in PCT-3(P＞0.05).The invasion was inhabited significantly by VEGF siRNA and bFGF siRNA in the three cell lines(P＜0.05).Combination of VEGF siRNA and bFGF siRNA resulted in more efficient influence in inhibition of invasion in Panc-1 and proliferation in PCT-3 and SW1990 than VEGF siRNA or bFGF siRNA individually.Conclusion The decreased expression of VEGF
VEGF siRNA及bFGF siRNA对胰腺癌细胞增殖和侵袭性的影响
