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EFFECT OF SURFACTANT SDS ON DETERMINATION OF NUCLEIC ACID WITH TERBIUM (III)FLUO

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EFFECT OF SURFACTANT SDS ON DETERMINATION OF NUCLEIC ACID WITH TERBIUM (III)FLUO

WuHui;WanYu

【期刊名称】《南京航空航天大学学报:英文版》 【年(卷),期】2002(019)001 【

The

effect

of

an

anionic

surfactant(sodium

dodecylsulfate.SDS)on the fluorescence properties of nucleic acid with terbium(III)is studied.Results show that ri-bonucleir acid (RNA)presents fluorescence

reaction

with

Tb(III)directly.but

deoxyribonucleic

acid(DNA)pre-sents similar fluorescence reaction only after its denatura-tion.In the presence of SDS ,the fluorescence intensity is 4.0 times and 3.5 times greater than that of DNA and RNA without SDS. 【总页数】4页(P.94-97)

【关键词】fluorescence;properties;gurfactant;DNA;RNA;terbium;荧光性质;表面活性剂;十二烷基磺酸钠;核酸;DNA;RNA;铽 【作者】WuHui;WanYu 【

CollegeofMaterialScienceandTechnology,NUAA29YudaoStreet,Nanjing210016,P.R.China 【正文语种】英文 【中图分类】O657.39 【相关文献】

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2.Fluorescence Quenching Investigation for Janus Green B and used as Probe in Determination of Nucleic Acids [J], 陈莉华; 刘六战; 沈含熙 3.Nucleic Acid Determination and Risk Assessment of Brucella in Milk Products [J], Bai Bi[1]; Fang Ying[1]; Lin Lizhong[2]; Xu Chunzhi[1]; Yang Feng[1]; Chen Hong[1]; Peng Yi[1]; Yuan Cuixia[1] 4.专题七 遗传的物质基础与基因工程 [J],

5.组织固定对核酸含量及完整性的影响 [J], 齐妍; 常彬; 李锋

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