HPLC法筛查骨刺片中的金胺O
张舒
【期刊名称】《中国药师》 【年(卷),期】2015(000)001
【摘要】目的：：筛查骨刺片中金胺 O。方法：采用 HPLC 法筛查骨刺片中金胺 O。色谱柱：Diamonsil -C18柱(250 mm ×4.6 mm,5μm)；流动相：乙腈-0.025 mol·L-1磷酸二氢钾(含0.2%三乙胺,并用磷酸调节pH至3.0)(35∶65)；流速：0.8 ml· min-1；检测波长：432 nm；柱温：35℃；进样量：10μl。结果：金胺O 的检测限为0.5 ng。经检测的45批市售品,有7批含有金胺O。结论：该方法简便、快速、准确,为保证该制剂的用药安全提供了依据
。%Objective:To
screen
auramine
O
in
Guci
tablets.
Methods:Auramine O was detected by HPLC method on a Diamon-sil-C18(250mm×4.6mm,5μm)columnwiththemobilephaseofacetonitrile-0.025mol·L-1monopotassiumphosphatebuffersolu-tion(containing 0. 2% triethylamine,adjusting pH to 3. 0 with phosphoric acid)(36∶65)at the flow rate of 0. 8 ml·min-1. The detec-tion wavelength was 432 nm and the column temperature was 35℃. Results:The LOD of auramine O was 0. 05ng. Among 45 batches of tested samples, auramine O was found in seven ones. Conclusion:The method is simple, rapid and accurate, which can guarantee the drug safety of Guci tablets. 【总页数】2页(172-173)
【关键词】高效液相色谱法;骨刺片;金胺O;筛查
HPLC法筛查骨刺片中的金胺O
