成纤维细胞生长因子21表达抑制对ApoE基因敲除小鼠糖
脂代谢的影响
罗妤;邓广江;杨刚毅;李伶;张莉莉;王波;GuntherBoden;李钶;李生兵
【期刊名称】《中华内分泌代谢杂志》 【年(卷),期】2011(027)012
【摘要】Objective To investigate the in vivo effects of down-regulating the FGF-21 gene expression by shRNA on glucose and lipids metabolism in high fat diet (HFD) fed ApoE-/- mice.Methoeds Male ApoE-/- mice were randomly divided into chow diet (CF)fed group ( NF,n =10),CF fed + pAd-shFGF-21 group ( NFG,n =9),and HFD fed group ( HF,n =10),HFD fed + Adv-null vector ( pAd-GFP ) group ( GFP,n =6) and HFD fed + pAd-shFGF-21 group ( HFG,n =10).Mice were fed for 16 weeks.C57BL/6J mice were set as control group ( NC group,n=10).NFG,HFG,and GFP groups were injected with pAd-shFGF-21or pAd-GFP by tail vein at the end of 15 weeks.The insulin sensitivity and glucoselipid metabolism were assessed by the hyperinsulinemic- euglycemic clamp technique using 3-[ 3 H] glucose as a tracer at the end of 16 week.Results The plasma FGF-21 levels in NFG and HFG groups were significantly degraded than those in NF and HF groups(20％-27％,P＜0.05),respectively.In the basal state,the fasting blood glucose,fasting
plasma
insulin,free
fatty-acids,triglycerides,total
cholesterol,and LDL-C were significantly higher,while the HDL-C was
成纤维细胞生长因子21表达抑制对ApoE基因敲除小鼠糖脂代谢的影响
