Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncu

Comparative analysis of proteomic profiles

between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

Yang Wang1?,Chao Wang1?,Zhuocheng Hou2,Kai Miao1,Haichao Zhao1,Rui Wang1,Min Guo1,Zhonghong Wu3, Jianhui Tian1and Lei An1*

【摘 要】Abstract：The endometrium of sheep consists of plenty of raised aglandular areas called caruncular(C),and intensely glandular intercaruncular areas(IC).In order to better understand the endometrium involved mechanisms of implantation,we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period,and then compared the proteomic profiles between these two areas.We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively.By comparing the proteome of these two areas, we found 170 differentially expressed proteins(DEPs)(P＜0.05),functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue,cell adhesion and protein transport,and so on. Our study,for the first time,provided a proteomic reference for elucidating the differences between C and IC areas,as an integrated function unit respectively,during the peri-implantation period.The results could help