羟基喜树碱对人胚肺成纤维细胞增殖与凋亡的影响
姜瑞姣;郑洁;刘良徛;李琳;徐淑睿
【期刊名称】《实用医学杂志》 【年(卷),期】2011(027)024
【摘要】Objectives To investigate the effects of hydrocamptothecin (HCPT) on the proliferation and apoptosis of human fetal lung fibroblasts cell line HFL1 in vitro. Methods Human fetal lung fibroblasts cell line HFL1 were cultured in vitro, and exposed to HCPT with different final concentrations (0.008-32 mg/L respectively) , cells without HCPT exposure served as the control. The cell proliferation was assessed by MTT colorimetric assay. The morphological change of the HFL1 were observed with light microscopy. The rate of cell apoptosis was identified by FCM. Results MTT assay indicated that HCPT significantly inhibited the proliferation of HFL1 in a dose-and time-dependent manner, apoptosis rates were significantly higher than that of control group (P < 0.01). After treatment with the concentration of 0.5 mg/L for 48 h, typical apoptotic morphological features of cell shrinkage , nucleoli disappearance and condensation and margination of nuclear chromatin were observed by TEM. Conclusion HCPT could significantly inhibit the proliferation of HFL1 in a dose-and time-dependent manner, and could induce apoptosis of HFL1 in vitro.%目的:探讨羟基喜树碱(HCPT)对体外培养的人胚肺成纤维细胞(HFL1)增殖与凋亡的影响.方法:常规培养HFLl细胞,以未加
羟基喜树碱对人胚肺成纤维细胞增殖与凋亡的影响
