外源激素配比对樱桃叶片再生体系的影响
侯思宇;孙朝霞;王玉国
【期刊名称】《山西农业大学学报（自然科学版）》 【年(卷),期】2011(031)002
【摘要】Prunus a vium L mass production is low limited by cutting propagation, so establishment of the rapid propagation system by cherry tissue culture plays a key role in solving this problem. The effect of three plant hormone proportions (BA, TDZ and IBA) on the in vitro cultured leaf regeneration system was investigated with orthogonal test design. The callus and shoots ratio of leaves was analyzed by intuitive, variance and effect analysis, and the soluble protein content of leaf callus were tested. The result showed that BA concentration variation had a remarkable effect on the callus and shoots ratio of leaves. TDZ and IBA had no effect on the ratio. Among the three plant hormones, the effect of callus rating was BA＞IBA＞TDZ; and the effect of shoots rating was BA＞TDZ＞IBA. The best culture medium of inducible leaf regeneration was combination 13 medium: MS+ BA 2. O mg · L-1 +TDZ 2. O mg · L-1 + IBA 2. O mg ·L-1. The soluble protein content of inducible leaf regeneration was 21. 17 g · L-1 in the combination 13 medium. This research succeeded in constructing leaf regeneration and screening the best plant hormone proportions (combination 13). That proportion was favorable for advancing protein metabolism and
外源激素配比对樱桃叶片再生体系的影响
